A rapid detection method of replication-competent plasmid DNA from COVID-19 mRNA vaccines for quality control

URL

https://jhss.scholasticahq.com/article/127890-a-rapid-detection-method-of-replication-competent-plasmid-dna-from-covid-19-mrna-vaccines-for-quality-control

Authors

Tyler J. Wang, Alex Kim, and Kevin Kim

Published Date

December 29, 2024

Publication

Journal of High School Science

Key Excerpts

"Abstract

... Here, we report a simple method to detect residual replication-competent plasmid DNA that is present in mRNA vaccines as impurities...

Discussion

Not more than 10 ng/dose... is a limit of cell substrate derived residual DNA recommended by the WHO guideline for parenteral viral vaccines...

In this study, residual DNA was detected from six vials of two different lots of Pfizer COVID-19 mRNA vaccines. The estimated amount of residual DNA in one human dose appears to be 6 to 470 times 10 ng."

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Medium

document

Topics

COVID-19,reverse transcription,vaccine composition,vaccine ingredients,vaccine quality control,vaccines