"Abstract
... Vaccination with 1-methylΨ (pseudouridine enriched) mRNA can elicit cellular immunity to peptide antigens produced by +1 ribosomal frameshifting in major histocompatibility complex-diverse people. The translation of 1-methylΨ mRNA using liquid chromatography tandem mass spectrometry identified nine peptides derived from the mRNA +1 frame. These products impact on off-target host T cell immunity that include increased production of new B cell antigens with far reaching clinical consequences. As an example, a highly significant increase in heart muscle 18-flourodeoxyglucose uptake was detected in vaccinated patients up to half a year (180 days). This review article focuses on medical biochemistry, proteomics and deutenomics principles that explain the persisting spike phenomenon in circulation with organ-related functional damage even in asymptomatic individuals. Proline and hydroxyproline residues emerge as prominent deuterium (heavy hydrogen) binding sites in structural proteins with robust isotopic stability that resists not only enzymatic breakdown, but virtually all (non)-enzymatic cleavage mechanisms known in chemistry...
Summary and Conclusions
Trends in mRNA vaccine and medicine development efforts ignore many basic principles of medical biochemistry, physiology, proteomics and deutenomics. Although in vitro/vivo–transcribed mRNAs encoding clinically important proteins have broad potentials for therapeutic applications, mRNA modified by pseudouridination and other changes, including methylation, is infeasible for clinical use because of its long-lasting and potentially permanent and immunostimulatory nature. Nucleoside modification is believed to be an effective approach to enhance stability by making the product resistant to ribonucleases. As a result, there is increased translational capacity of mRNA while potentially diminishing its immunogenicity in vivo. The persistent nature of mRNA coding for SARS-CoV-2 spike protein provides a dangerously long exposure to an unlimited dose of this pathogenic protein, and thus, it needs re-evaluation for continued human use. We have provided the molecular basis for a wide distribution of injuries, disabilities, and deaths resulting from spike protein-related diseases, which derive from ill-advised continued use of these products. Understanding the above overarching proteomics and deutenomics mechanisms, especially in cell growth and transformation, in modified mRNA vaccines-related severe adverse events is necessary for a scientifically informed benefit/risk evaluation of such vaccinations."
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