"Abstract
This study examines the persistence of synthetic mRNA from the COVID-19 vaccine Comirnaty in the blood, placenta, sperm, and seminal fluid of both vaccinated and unvaccinated individuals. Samples were collected from 34 participants, including 22 pregnant women, four male patients from a fertility clinic (providing eight samples), and eight additional individuals. RNA was extracted and analyzed using nested PCR, and the resulting amplicons were confirmed by Sanger sequencing...
Discussion
... The use of nested PCR in the present study enabled us to achieve much higher sensitivity, and reliably detect the vaccine mRNA in placenta, albeit non-quantitively...
We also detected vaccine mRNA in the sperm of all three vaccinated donors who produced sperm. Interestingly, this contrasts with the presence of the SARS-COV-2 itself...
We detected vaccine mRNA in the blood, placenta and semen of some subjects over 200 days post-immunization. The use of nested PCR with 20 and 35 cycles in the first and second PCRs, respectively, combined with relatively large amount of RNA used for analysis, enabled detection of even very few vaccine molecules...
The encapsulation of the mRNA within nanoparticles, together with the modifications to the RNA nucleotides, appear to bestow enhanced stability on the vaccine mRNA."
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